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John Louis I, Prince of Anhalt-Dornburg (4 May 1656, in Zerbst – 1 November 1704, in Dornburg), was a German prince of the House of Ascania and ruler of the principality of Anhalt-Dornburg, as well as a grandfather of Catherine the Great.

He was the sixth (but fourth surviving) son of John VI, Prince of Anhalt-Zerbst, by his wife Sophie Auguste, daughter of Frederick III, Duke of Schleswig-Holstein-Gottorp.